| Título: | Development of analytical methodologies to assess recalcitrant pesticide bioremediation in biobeds at laboratory scale |
| Autores: | ANISLEIDY RIVERO, Autor ; SILVINA NIELL, Autor ; HORACIO HEINZEN, Autor ; MARÍA PÍA CERDEIRAS, Autor ; MARÍA VERÓNICA CESIO, Autor |
| Tipo de documento: | documento electrónico |
| Fecha de publicación: | 2016 |
| ISBN/ISSN/DL: | 68609 |
| Dimensiones: | pp.17-22 |
| Nota general: | En: Talanta, 2016, 1(153) |
| Langues: | Inglés |
| Clasificación: | |
| Resumen: | To assess recalcitrant pesticide bioremediation it is necessary to gradually increase the complexity of the biological system used in order to design an effective biobed assembly. Each step towards this effective biobed design needs a suitable, validated analytical methodology that allows a correct evaluation of the dissipation and bioconvertion. Low recovery yielding methods could give a false idea of a successful biodegradation process. To address this situation, different methods were developed and validated for the simultaneous determination of endosulfan, its main three metabolites, and chlorpyrifos in increasingly complex matrices where the bioconvertor basidiomycete Abortiporus biennis could grow. The matrices were culture media, bran, and finally a laboratory biomix composed of bran, peat and soil. The methodology for the analysis of the first evaluated matrix has already been reported. The methodologies developed for the other two systems are presented in this work. The targeted analytes were extracted from fungi growing over bran in semisolid media YNB (Yeast Nitrogen Based) with acetonitrile using shaker assisted extraction, The salting-out step was performed with MgSO4 and NaCl, and the extracts analyzed by GC-ECD. The best methodology was fully validated for all the evaluated analytes at 1 and 25 mg kg−1 yielding recoveries between 72 and 109% and RSDs |
| Creative Commons : | CC BY-NC-ND |
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