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A rapid and simple method for the determination of taurine (2-aninoethanesulphonic acid) in complex samples is described. It is based on the HPLC separation of the dinitrophenyl (DNP) derivative of taurine. The reaction conditions are selected t[...]texto impreso
Taurine (2-aminoethanesulfonic acid) was quantitated by reversed-phase chromatography on a C18. Resolve column using a linear gradient of 9-l 1% methanol in water. Glutamine was used as the internal standard. Pre-column derivatization of the am[...]