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Designer variable repeat length polypeptides as scaffolds for surface immobilization of quantum dots
MEDINTZ, IGOR L. ; SAPSFORD, KIM E. ; CLAPP, AARON R. ; PONS, THOMAS ; HIGASHIYA, SEIICHIRO ; WELCH, JOHN T. ; MATTOUSSI, HEIDIWe demonstrate the use of a series of engineered, variable-length de noVo polypeptides to discretely immobilize luminescent semiconductor nanocrystals or quantum dots (QDs) onto functional surfaces. The polypeptides express N-terminal dicysteine[...]
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SUSUMU, KIMIHIRO ; UYEDA, H. TETSUO ; MEDINTZ, IGOR L. ; PONS, THOMAS ; DELEHANTY, JAMES B. ; MATTOUSSI, HEIDIWe have designed and synthesized a series of modular ligands based on poly (ethylene glycol) (PEG) coupled with functional terminal groups to promote water-solubility and biocompatibility of quantum dots (QDs). Each ligand is comprised of three [...]
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Luminescent semiconductor quantum dots (QDs) have great potential for use in biological assays and imaging. These nanocrystals are capped with surface ligands (bifunctional molecules, amphiphilic polymers, phospholipids, etc.) that render them h[...]
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PONS, THOMAS ; MEDINTZ, IGOR L. ; SAPSFORD, KIM E. ; HIGASHIYA, SEIICHIRO ; GRIMES, AMI F. ; ENGLISH, DOUGLAS S. ; MATTOUSSI, HEIDILuminescent quantum dots (QDs) were proven to be very effective fluorescence resonance energy transfer donors with an array of organic dye acceptors, and several fluorescence resonance energy transfer based biosensing assemblies utilizing QDs ha[...]
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MEDINTZ, IGOR L. ; BERTI, LORENZO ; PONS, THOMAS ; GRIMES, AMI F. ; ENGLISH, DOUGLAS S. ; ALESSANDRINI, ANDREA ; FACCI, PAOLO ; MATTOUSSI, HEIDISelf-assembly of proteins, peptides, DNA, and other biomolecules to semiconductor quantum dots (QD) is an attractive bioconjugation route that can circumvent many of the problems associated with covalent chemistry and subsequent purification. Po[...]
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DELEHANTY, JAMES B. ; MEDINTZ, IGOR L. ; PONS, THOMAS ; BRUNEL, FLORENCE M. ; DAWSON, PHILIP E. ; MATTOUSSI, HEIDIWe demonstrate the use of self-assembled luminescent semiconductor quantum dot (QD)-peptide bioconjugates for the selective intracellular labeling of several eukaryotic cell lines. A bifunctional oligoarginine cell penetrating peptide (based on [...]
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We present a single particle fluorescence resonance energy transfer (spFRET) study of freely diffusing self-assembled quantum dot (QD) bioconjugate sensors, composed of CdSe-ZnS core-shell QD donors surrounded by dye-labeled protein acceptors. W[...]
