| Título: | Journal of Liquid Chromatography & Related Technologies |
| Autores: | PAPADOYANNIS, I. N., Autor ; TSIONI, G. K., Autor ; SAMANIDOU, V. F., Autor |
| Tipo de documento: | documento electrónico |
| Fecha de publicación: | 1997 |
| ISBN/ISSN/DL: | 68054 |
| Nota general: | Donado por autor. |
| Langues: | Inglés |
| Clasificación: | |
| Resumen: |
An automated reversed phase high performance liquid-chromatography (W-HPLC) method is described, for the simultaneous analysis of water soluble [ascorbic acid (C).nicotinic acid, nicotinamide. folic acid, cyanocobalaniine (BIZ).and riboflavin(B?)]and fat soluble (retinol. a-tocopherol. a-tocopherol acetate) vitamins. The compounds are separated after solid-phase extraction (SPE) on C, cartridges, where water soluble vitamins pass unretained, while fat soluble vitamins are retained on the sorbent. After isolation of the two fractions, water soluble vitamins are separated on a Lichrosorb RP-18 250~4.0 mm. 5 pm analytical column, using a gradient elution system consisting of CH30H- 0.05 M CH3COONH, ( 5:95 v/v ) changing to ( 30:70 v/v ) over
a period of 20 min at a flow rate 1 mL/min. Fat soluble vitamins 3203 Copyriglit Q 1997 by Marcel Dekker, Inc. Downloaded By: [HEAL- Link Consortium] At: 09:06 19 June 2007 3204 PAPADOYANNIS ET AL. are separated on 21 Spherisorb RP-18 220x4 6 mm. 5 pm analjticd column kvith an isocratic mobile phase consisting of CHIOH-CHICN ( 30 70 1/11 ) at a flow rate of 1 5 mL/min A UV-\ IS detector operated at 270 nni and 290 nm for water 5oluble and fat soluble Litamins. respectively. IS used for detection and quantitation of the analytes Xanthine is used as internal standard at a concentration of 4 2ng/L for nater solublc kitamins. while anlhraquinone is used as internal standard at a concentration of 3 5 ng/mL for fat soluble vitamin\ Linearie is observed up to 10ngimL for ascorbic acid, folic acid. and riboflavin. up to 15 ng/mL for nicotinic acid nicotinamide. and cyanocobalamine, while up to 20 ng/mL for all fat soluble vitamins. Limits of detection ranged from 2.5 - 5.0 ng for water soluble vitamins and 2.0 - 5.0 ng for fat soluble vitamins. Method’s \didation is achieved in terms of day-to-day and within-day reproducibility studies. Long term stability study is conducted during routine operation of the system over a period of ten consecutive days. The developcd method is applied to the analysis of pharmaceutical preparations: (tablets, injection solutions) and biological fluids:(blood serum. urine). SPE technique is used for the isolation of the vitamins from the matrix of human biological fluids: blood serum (40 pL) and urine (100 pL). High extraction reco1,eries are achieved using Merck Lichrolut RP- 18 cartridges. |
| Nota de contenido: | En: J. LIQ. CHROM. & EL. TECHNOL.-- 1997, 20( 19): 3203-323 |
