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Título:
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Mass Spectral Analysis of some Derivatives and in vitro Metabolites of Stevio of the Natural Sweeteners, Stevioside, Rebaudioside Al, the Aglycone and Rubusoside / Compadre, C. M.
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Autores:
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C. M. COMPADRE, Autor ;
R. A. HUSSAIN, Autor ;
N. P. NANAYAKKARA, Autor ;
J. M. PEZZUTO, Autor ;
A. D. KINGHORN, Autor
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Tipo de documento:
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documento electrónico
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Fecha de publicación:
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1988
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ISBN/ISSN/DL:
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68019
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Nota general:
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En: Biomedical and Environmental Mass Spectrometry.-- 1988, 15: 211-222
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Langues:
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Inglés
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Clasificación:
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EDULCORANTES
ESPECTROMETRÍA DE MASA
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Resumen:
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Steviol (ent-13-hydroxykaur-16-en-19-oic acid), the aglycone of various plant-derived glycoside sweeteners consumed by human populations, is known to be mutagenic toward Salmonella tymphimurium strain TM677 when metabolically activated using a 9000 x g supernatant fraction derived from the liver of Aroclor 1254-pretreated rats. Mass spectral analysis of this diterpenoid and some analogs revealed characteristic patterns reflecting differential stereochemistry at the C/D rings and variations in the nature of the substituents present. Such information has been used to help identify several in vitro metabolites of steviol in conditions known to produce a mutagenic response, when analyzed by gas chromatography/mass spectrometry. The major pathways of such steviol mammalian metabolism proved to be allylic oxidation and epoxidation. 15-Oxosteviol, a product of oxidation of the major steviol metabolite, 15alpha-hydroxysteviol, was found to be a direct-acting mutagen [corrected].
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