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Título:
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Determination of caseinomacropeptide with capillary zone electrophoresis and its application to the detection and estimation of rennet whey solids in milk and buttermilk powder
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Autores:
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OLIEMAN, CORNELIS ;
RIEL, JAN VAN
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Tipo de documento:
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texto impreso
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ISBN/ISSN/DL:
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65220
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Langues:
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Inglés
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Clasificación:
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ELECTROFORESIS
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Resumen:
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The presence of rennet whey solids in milk powder and buttermilk powder could be detected by analyzing the caseinomacropeptide (6.7 kDa) content by capillary zone electrophoresis. A hydrophilically coated capillary was used in combination with 6 M urea in a citrate buffer at low pH. Under these conditions, genetic A and B variants migrated as a single peak. This afforded a detection limit of 0.4% of rennet whey solids in combination with large volume injections and on-column isotachophoretic concentration of the sample. The detector response (UV at 214 nm) was linear in the range of 1-20% rennet whey solids and the recovery of caseinomacropeptide was 98%. Pseudo-caseinomacropeptide, lacking the N-terminal Met-residue, could be separated from caseinomacropeptide, thus preventing false positive results for some types of acid buttermilk powder.
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Nota de contenido:
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En: Electrophoresis. --1995, 16: 529-533
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