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Título:
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Analysis of folate form distribution by affinity followed by reversed-phase chromatography with electrochemical detection
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Autores:
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BAGLEY, PAMELA J. ;
SELHUB, JACOB
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Tipo de documento:
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texto impreso
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ISBN/ISSN/DL:
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38960
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Nota general:
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ISBN:
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Langues:
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Inglés
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Clasificación:
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CROMATOGRAFÍA LÍQUIDA
FOLATOS
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Resumen:
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Naturally occurring folates exist in multiple forms, differing in pteridine ring structure and number of glutamate residues. The ability to measure these folate coenzymes in tissues and cells gives important information about in vivo folate metabolism. Folates were heat-extracted from biological samples. A two-column HPLC system with four-channel coulometric electrochemical detection was used for analysis. An affinity column was used first to purify folates from the extract. Purified folates were eluted from the affinity column onto a phenyl analytical column, utilizing a switching valve, and folate forms were separated using an acetonitrile gradient.
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Nota de contenido:
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En: Clinical Chemistry. -- 46 (3) : 404-411 (2000)
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