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Título:
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Self-assembled quantum dot-peptide bioconjugates for selective intracellular delivery
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Autores:
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DELEHANTY, JAMES B. ;
MEDINTZ, IGOR L. ;
PONS, THOMAS ;
BRUNEL, FLORENCE M. ;
DAWSON, PHILIP E. ;
MATTOUSSI, HEIDI
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Tipo de documento:
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texto impreso
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ISBN/ISSN/DL:
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41261
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Nota general:
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ISBN:
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Langues:
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Inglés
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Clasificación:
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FLUORESCENCIA
NANOTECNOLOGÍA
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Resumen:
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We demonstrate the use of self-assembled luminescent semiconductor quantum dot (QD)-peptide bioconjugates for the selective intracellular labeling of several eukaryotic cell lines. A bifunctional oligoarginine cell penetrating peptide (based on the HIV-1 Tat protein motif) bearing a terminal polyhistidine tract was synthesized and used to facilitate the transmembrane delivery of the QD bioconjugates. The polyhistidine sequence allows the peptide to self-assemble onto the QD surface via metal-affinity interactions while the oligoarginine sequence allows specific QD delivery across the cellular membrane and intracellular labeling as compared to nonconjugated QDs. This peptide-driven delivery is concentration-dependent and thus can be titrated. Upon internalization, QDs display a punctate-like staining pattern in which some, but not all, of the QD signal is colocalized within endosomes. The effects of constant versus limited exposure to QD-peptide conjugates on cellular viability are evaluated by a metabolic specific assay, and clear differences in cytotoxicity are observed. The efficacy of using peptides for selective intracellular delivery is highlighted by performing a multicolor QD labeling, where we found that the presence or absence of peptide on the QD surface controls cellular uptake.
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Nota de contenido:
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En: Bioconjugate Chemistry. -- (17) : 920-927 (2006)
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