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Título:
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Determination of ascorbic and dehydroascorbic acid in potatoes (solanum tuberosum) and strawberries using ion-exclusion chromatography
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Autores:
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GRAHAM, WILLIAM D. ;
ANNETTE, DONALD
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Tipo de documento:
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texto impreso
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ISBN/ISSN/DL:
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45180
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Nota general:
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Se posee también versión en papelISBN:
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Langues:
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Inglés
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Clasificación:
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ÁCIDO ASCÓRBICO
CROMATOGRAFÍA LÍQUIDA
FRUTILLAS
PAPAS
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Resumen:
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A high-performance liquid chromatographic method has been developed for determination of ascorbic and dehydroascorbic acid. Samples were extracted with 62.5 mM metaphosphoric acid and ascorbic acid determined using an ion-exclusion column with detection at 245 nm. Dehydroascorbic acid was determined after reduction to ascorbic acid. The use of ion-exclusion chromatography enables ascorbic acid to be completely resolved from co-extracted material in both raw and cooked foods. Completeness of separation was confirmed using a photodiode-array detector.
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Nota de contenido:
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En: Journal of Chromatography. -- (592) : 187-194 (1992)
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