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Título:
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Determination of cyanocobalamin in foods by high performance liquid chromatography with visible detection after solid phase extraction and membrane filtration for the precolumn separation of lipophilic species
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Autores:
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ONO, ICHIRO ;
IWASE, HIROSHI
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Tipo de documento:
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texto impreso
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ISBN/ISSN/DL:
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32192
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Nota general:
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ISBN:
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Langues:
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Inglés
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Clasificación:
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ANÁLISIS DE ALIMENTOS
MUESTREO
VITAMINAS
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Resumen:
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Determination of trace amounts of cyanocobalamin (18 ng/g) in fatty foods was performed by solid-phase extraction and high-performance liquid chromatography (HPLC) with visible detection at 550 nm using a membrane filter for the precolumn separation of particulate material. It was found that a membrane filter (HLC-Disk 25, 0.45 ym) was most suitable for the separation of oily particulates. A sample solution was applied to a solid-phase extraction cartridge and then cyanocobalamin was eluted using a 50% aqueous acetonitrile solution followed by HPLC. This method was suitable for the determination of trace amounts of cyanocobalamin in nutrient samples. The proposed method was simple, rapid (extraction time: ca. 12 min, analysis time: ca. 12 min), sensitive (detection limit: ca. 0.15 ng at a signal-to-noise ratio of 3:1), highly selective and reproducible (relative standard deviation: 2.67%) for cyanocobalamin. The calibration graph for cyanocobalamin was linear in the range of 0.1 to 30 ng. Recovery of cyanocobalamin was over 90% by the standard addition method.
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Nota de contenido:
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En: Journal of Chromatography A . -- (771) : 127-134 (1997)
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