Título:
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Detection of shiga toxin–producing Escherichia coli O26, O45, O103, O111, O113, O121, O145, and O157 serogroups by multiplex polymerase chain reaction of the wzx gene of the O-antigen gene cluster
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Autores:
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CHITRITA DEBROY, Autor ;
ELISABETH ROBERTS, Autor ;
ÁNGELA, M. VALADEZ, Autor ;
EDWARD G. DUDLEY, Autor ;
CATHERINE N. CUTTER, Autor
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Tipo de documento:
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documento electrónico
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ISBN/ISSN/DL:
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68669
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Nota general:
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Foodborne Pathogens and Disease, 2011, 8(5), pp.651-652
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Langues:
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Inglés
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Clasificación:
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CONTAMINACIÓN DE ALIMENTOS
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Resumen:
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O-antigens on the surface of Escherichia coli are important virulence factors that are targets of both the innate and adaptive immune system and play a major role in pathogenicity. O-antigens that are responsible for antigenic specificity of the strain determine the O-serogroup. E. coli O26, O45, O103, O111, O113, O121, O145, and O157 have been the most commonly identified O-serogroups associated with Shiga toxin–producing E. coli (STEC) implicated in outbreaks of human illness all over the world. A multiplex polymerase chain reaction assay was developed to simultaneously detect the eight STEC O-serogroups targeting the wzx (O-antigen-flippase) genes of all O-antigen gene clusters. The sensitivity of the multiplex polymerase chain reaction was found to be 10 colony forming units for each O-group when enriched in broth and 100 colony forming units when enriched in artificially inoculated apple juice diluted with tryptic soy broth for 16 h at 37°C. The method can be used for detecting STEC O-groups simultaneously and may be exploited for improving the safety of food products.
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